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Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry
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Neuroscience
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JoVE Journal Neuroscience
Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

DOI:
10.3791/56538-v

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12:34 min

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December 06, 2017

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, ,
1Department of Medicine I and Clinical Chemistry,University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich,Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD)

Chapters

  • 00:05Title
  • 01:28Dissection of Sciatic Nerve and Dorsal Root Ganglion
  • 04:02Digestion of Sciatic Nerve and Dorsal Root Ganglion
  • 06:36Setup and Flow Cytometry and Running of Samples
  • 10:00Results: Flow Cytometry Analysis of Surface Expressed Markers on Singlets from the Sciatic Nerve and Dorsal Root Ganglion
  • 11:46Conclusion

Summary

Automatic Translation

Automatic Translation

Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.

Tags

Keywords: Flow CytometryImmune CellsSciatic NerveDorsal Root GanglionNeuropathyNeuroinflammationMouseDissectionCell IsolationDigestion

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