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Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain
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Neuroscience
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JoVE Journal Neuroscience
Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

DOI:
10.3791/56547-v

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09:50 min

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October 29, 2017

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,
1Department of Biology, Chemistry and Environmental Sciences,American University of Sharjah, 2Department of Biology,The City College of New York

Chapters

  • 00:05Title
  • 01:03Preparatory Steps
  • 02:20Animal Treatment, Tissue Preparation, and Staining
  • 04:21Synaptic Zinc Histochemistry
  • 06:06Densitometry
  • 07:24Results: Histochemical Technique to Study Synaptic Zinc Staining in Adult Ferret Brain
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a histochemical procedure that reveals characteristic laminar and areal zinc staining patterns in different brain regions. The zinc-staining pattern may be used in conjunction with other anatomical markers to reliably distinguish layers and regions in the developing and adult brain.

Tags

Keywords: Synaptic Zinc HistochemistryBrain RegionsBrain LaminaeDeveloping BrainAdult BrainFerretVisual CortexSodium SeleniteHydroquinoneSilver LactateTranscardial PerfusionParaformaldehydeSucrose Fixation

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