Journal
/
Neuroscience
/
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

DOI:
10.3791/56553-v

•

06:16 min

•

March 28, 2018

•

,
1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Chapters

  • 00:04Title
  • 00:58Preparation of Cortical Slices
  • 02:24Procedure of Electroporation
  • 04:37Results: Thalamocortical Axons Coculture and Time-lapse Imaging
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. Individual thalamocortical axons and their presynaptic terminals are visualized by a single cell electroporation technique with DsRed and GFP-tagged synaptophysin.

Tags

Keywords: ThalamocorticalAxon BranchingSynapse FormationOrganotypic CoculturesNeural Circuit FormationAxon GrowthSynapse FormationCerebral CortexPostnatal Day-two RatPrimary Visual CortexSomatosensory CortexCulture InsertThalamic BlockE15 EmbryoGlass CapillariesElectrode PipettePlasmid Injection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56553?article_type=v

Automated Interactive Video Playback for Studies of Animal Communication
check_url/56553?article_type=v

Visualization of Proprioceptors in Drosophila Larvae and Pupae
check_url/56553?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/56553?article_type=v

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
check_url/56553?article_type=v

Whole-mount Imaging of Mouse Embryo Sensory Axon Projections
check_url/56553?article_type=v

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
check_url/56553?article_type=v

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
check_url/56553?article_type=v

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
check_url/56553?article_type=v

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
check_url/56553?article_type=v

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

Read Article