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Neuroscience

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Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
 

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

Article doi: 10.3791/56553
March 28th, 2018 Usage Statistics

Summary March 28th, 2018

This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. Individual thalamocortical axons and their presynaptic terminals are visualized by a single cell electroporation technique with DsRed and GFP-tagged synaptophysin.

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