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Cancer Research
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向上移动: 一个简单而灵活
的体外
三维入侵检测协议
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Moving Upwards: A Simple and Flexible
In Vitro
Three-dimensional Invasion Assay Protocol
Please note that all translations are automatically generated.
Click here for the English version.
向上移动: 一个简单而灵活
的体外
三维入侵检测协议
DOI:
10.3791/56568-v
•
06:14 min
•
March 12, 2018
•
Tanner J. McArdle
,
Brenda M. Ogle
2,3,4,5
,
Felicite K. Noubissi
1
Department of Biomedical Engineering
,
University of Minnesota – Twin Cities
,
2
Stem Cell Institute
,
University of Minnesota – Twin Cities
,
3
Masonic Cancer Center
,
University of Minnesota – Twin Cities
,
4
Lillehei Heart Institute
,
University of Minnesota – Twin Cities
,
5
Institute for Engineering in Medicine
,
University of Minnesota – Twin Cities
,
6
Department of Biology
,
Jackson State University
Chapters
00:05
Title
01:11
Culture Plate, Collagen Gel Preparation, and Cell Imaging
03:33
Results: Representative Inverted Vertical Invasion Assay Analyses
04:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种倒置垂直入侵检测方法, 可用于量化三维环境中细胞的迁移和入侵能力, 同时保持细胞微环境。本试验适用于稀有和/或敏感细胞。
Tags
3D Invasion Assay
In Vitro Invasion Assay
Cell Migration
Cell Invasion
Cancer Research
Metastasis
Collagen Gel
Cell Culture
DAPI
Paraformaldehyde
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