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Methodology for Sputum Induction and Laboratory Processing
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JoVE Journal Medicine
Methodology for Sputum Induction and Laboratory Processing

Methodology for Sputum Induction and Laboratory Processing

DOI:
10.3791/56612-v

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13:28 min

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December 17, 2017

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, , , , , , , ,
1Department of Respiratory Medicine, CHU Liege, GIGA I3 Research Group,University of Liege, 2Department of Clinical Pharmacy, CIRM (Center for Interdisciplinary Research on Medicines),University of Liege

Chapters

  • 00:00Title
  • 01:10Sputum Induction
  • 05:27Sputum Processing
  • 10:34Results: Hemocytometer and Stained Cytospins
  • 12:31Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe the technique of sputum induction. This protocol also explains the sputum processing to perform a differential cell count and to collect sputum supernatant and cells for further analysis.

Tags

Keywords: Sputum InductionInduced SputumAirway DiseasePulmonary FibrosisNon-invasiveSpirometryBronchodilatorsHypertonic SalineIsotonic SalineSalbutamolNebulizerMedical SupervisionTidal Breathing

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