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Journal / Bioengineering / Geliştirilmiş 3D hidrojel Neuroinflammation Vitro …
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Bioengineering

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JoVE Journal Bioengineering
Geliştirilmiş 3D hidrojel Neuroinflammation Vitro modelleme için birincil gliyal hücreler kültürleri
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Geliştirilmiş 3D hidrojel Neuroinflammation Vitro modelleme için birincil gliyal hücreler kültürleri

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Burada, biz fare 3D kültür için bir protokol beyin kaynaklı glia hücreleri, astrocytes, microglia ve oligodendrocytes de dahil olmak üzere mevcut. Biz birincil hücre kültürü, methacrylated hyaluronik asit (HAMA) hidrojel sentezi, HAMAphoto polimerizasyon ve hücre saklamasını ve confocal ve tarama elektron mikroskobik görüntüleme için işleme örnek göstermektedir.

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Biyomühendislik sayı: 130 3D hücre kültürü hidrojel birincil glia astrocyte microglia oligodendrocyte neuroinflammation hyaluronik asit HAMA
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