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用 3 d-印刷模具制作细胞播种和组织环自组装用的自定义琼脂糖井
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds
DOI:

08:16 min

April 02, 2018

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Chapters

  • 00:05Title
  • 00:363D-printing the Mold and Casting the Polydimethylsiloxone (PDMS) Negative
  • 02:41Agarose Mold Fabrication
  • 04:01Seeding Tissue Rings with Rat Aortic Smooth Muscle Cells (RaSMCs)
  • 06:56Results: Self-assembled Tissue Rings
  • 07:36Conclusion

Summary

Automatic Translation

该协议描述了一个平台, 用于制造可变尺寸的自组装组织环使用定制的 3 d 印刷塑料模具。在 3 d-印刷模中固化了该底片;然后, 琼脂糖被投在固化的中和阴性。细胞被播种到产生的琼脂糖阱中, 它们聚集成组织环。

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