<em>In Vitro</em> SUMOylation Assay to Study SUMO E3 Ligase Activity

Wan-Shan Yang; Mel Campbell; Hsing-Jien Kung; Pei-Ching Chang

doi:10.3791/56629

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Biology

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In Vitro SUMOylation Assay te studeren SUMO E3 Ligase activiteit

<em>In Vitro</em> SUMOylation Assay to Study SUMO E3 Ligase Activity

JoVE Journal
Biology

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JoVE Journal Biology

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

In Vitro SUMOylation Assay te studeren SUMO E3 Ligase activiteit

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9,286 Views

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09:45 min

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January 29, 2018

DOI:

10.3791/56629-v

DOI:

10.3791/56629-v

•

09:45 min

January 29, 2018

•

1 Views

Wan-Shan Yang¹, Mel Campbell², Hsing-Jien Kung^2,3,4,5, Pei-Ching Chang^1,6

¹Institute of Microbiology and Immunology,National Yang-Ming University, ²UC Davis Cancer Center,University of California, Davis, ³Department of Biochemistry and Molecular Medicine,University of California, Davis, ⁴Institute for Translational Medicine, College of Medical Science and Technology,Taipei Medical University, ⁵Division of Molecular and Genomic Medicine,National Health Research Institutes, ⁶Center for Infectious Disease and Cancer Research,Kaohsiung Medical University

Transcript

Summary

In tegenstelling tot ubiquitin ligases, zijn paar E3 SUMO ligases geïdentificeerd. Deze gewijzigde in vitro SUMOylation protocol vermag roman SUMO E3 ligases identificeren door een reconstructie in vitro assay.

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In Vitro SUMOylation Assay te studeren SUMO E3 Ligase activiteit

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