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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

DOI:
10.3791/56631-v

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10:09 min

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January 26, 2018

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, , , ,
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine,University of Freiburg, 2Faculty of Biology,University of Freiburg

Chapters

  • 00:05Title
  • 00:49Isolation of Cerebellar Granular Neuron Progenitors (CGNP)
  • 03:59Cell Fixation and Chromatin Shearing
  • 05:53Preclearing
  • 06:47Preparation of Antibody-Coupled Beads
  • 07:37Chromatin Immunoprecipitation
  • 08:51Results: H3K79me2 ChIP-qPCR and ChIP-Seq Analysis
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

We present an effective and reproducible method to isolate and culture neural progenitor cells from embryonic and postnatal brain tissue for chromatin immunoprecipitation (ChIP) of histone 3 lysine 79 dimethylation (H3K79me2) - a histone mark located within the globular domain of histone 3.

Tags

Neural ProgenitorsChromatin ImmunoprecipitationHistone 3 Lysine 79 DimethylationCerebral CortexCerebellumBrain DevelopmentEpigenetic ModificationsIn VivoHistone ModificationsCell CultureCerebellar CellsAstrocytesPoly-D-lysine

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