Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

The overall goal of this procedure, is to demonstrate how to manually synthesize oligo-peptoids and how to analyze their monomer sequence by using mass spectrometry techniques. The method can help beginners to answer key questions in a peptoid field such as how to make peptoids and how to characterize their monomer sequence. The main advantage of this technique is that peptoids with special monomer residues can be synthesized and analyzed in the standard analytical chemistry lab.

Even though this method focuses on oligo-peptoids it can also be applied to other systems such as peptides and oligo-nucleotides. Generally individuals new to this technique will struggle because achieving optimal mass spectrometry conditions is difficult without visual demonstration. We first had the idea for this method when we wanted to characterize the structural features of peptoids by tandem mass spectrometry methods.