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Developmental Biology
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JoVE Journal Developmental Biology
A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes
 

A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes

Article doi: 10.3791/56668
March 3rd, 2018 •
1, 1, 1, 1,2, 1,3, 4, 1, 1
1Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani", University of Pavia, 2Institute of Biotechnology, University of Helsinki, 3Department of Reproductive Medicine, University of California San Diego, 4Dipartimento di Ingegneria Industriale e dell'Informazione, University of Pavia

Summary
Automatic Translation
March 3rd, 2018

Here, we present a protocol for non-invasive assessment of oocyte developmental competence performed during their in vitro maturation from the germinal vesicle to the metaphase II stage. This method combines time-lapse imaging with particle image velocimetry (PIV) and neural network analyses.

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