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Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
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Biochemistry
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JoVE Journal Biochemistry
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

DOI:
10.3791/56778-v

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08:16 min

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January 16, 2018

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, , , , , , ,
1Mass Spectrometry Core Facility,University of Sydney, 2Proteomics Core Facility,University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney

Chapters

  • 00:05Title
  • 00:29Preparation of Reagents and Equipment
  • 01:21Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
  • 02:40Tissue Digestion
  • 03:57Matrix Coating via Sublimation
  • 05:26Recrystallization and Instrumentation
  • 06:20Results: Correctly and Incorrectly Prepared Human Brain Tissue
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass spectrometry.

Tags

Keywords: Formalin FixedPeptide Mass Spectrometry ImagingNitrocellulose CoatingVapor ChamberXyleneEthanolCarnoy’s FluidTris BufferPressure CookerTrypsin

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