Journal
/
Medicine
/
A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

DOI:
10.3791/56780-v

•

07:41 min

•

February 20, 2018

•

, , , ,
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research,KU Leuven

Chapters

  • 00:05Title
  • 00:50Ca2+ Mobilization Assay Cell Seeding
  • 01:58Fluorescence Plate Reader Protocol Setup
  • 04:38Fluorescence Assay
  • 05:36Results: Representative Chemokine Ligand: Receptor Specificity Analyses
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

The described cellular assay is designed for the identification of CXC chemokine receptor 4 (CXCR4)-interacting agents that inhibit or stimulate, either competitively or allosterically, the intracellular Ca2+ release initiated by CXCR4 activation.

Tags

Keywords: G Protein-coupled ReceptorCalcium MobilizationFluorescence-based AssayAgonistAntagonistAllosteric ModulatorDrug DiscoveryIntracellular CalciumGPCRCell-based AssayHigh-throughput Screening

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56780?article_type=v

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
check_url/56780?article_type=v

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
check_url/56780?article_type=v

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
check_url/56780?article_type=v

Dynamic Visual Tests to Identify and Quantify Visual Damage and Repair Following Demyelination in Optic Neuritis Patients
check_url/56780?article_type=v

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
check_url/56780?article_type=v

A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics
check_url/56780?article_type=v

A Clinical Trial Assessing the Safety, Efficacy, and Delivery of Olive-Oil-Based Three-Chamber Bags for Parenteral Nutrition
check_url/56780?article_type=v

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
check_url/56780?article_type=v

A Simple and Effective Method to Consistently Isolate Mouse Cardiomyocytes
check_url/56780?article_type=v

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Read Article