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A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

DOI:
10.3791/56785-v

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10:36 min

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January 09, 2018

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, , , , , , , , ,
1Department of Anatomical and Cellular Pathology,The Chinese University of Hong Kong, 2Li Ka Shing Institute of Health Sciences and Department of Medicine & Therapeutics,The Chinese University of Hong Kong

Chapters

  • 00:05Title
  • 01:24Preparation of OP9 Conditional Medium
  • 02:55Isolation of Mouse Bone Marrow Cells
  • 06:14siRNA-mediated Gene Silencing of Differentiating Natural Killer Cells
  • 06:43Analysis of Natural Killer Cell Differentiation Using Flow Cytometry
  • 07:35Results: Study of siRNA Dependent Gene Silencing on the Differentiation of Bone Marrow Derived Natural Killer Cells in a Feeder-free In Vitro System
  • 09:27Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to mass-produce gene-silencing murine NK cells by using a feeder-free differentiation system for mechanistic study in vitro and in vivo.

Tags

Keywords: Feeder-free SystemMass ProductionMurine Natural Killer CellsIn VitroInnate ImmunologyNatural Killer Cell DevelopmentCancer ImmunityBone MarrowPersonal ImmunotherapyOP9 CellsAlpha-MEM MediumPhosphate-buffered SalinePentobarbitoneFemur Bones

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