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Journal
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Immunology and Infection
/
报告酶荧光法检测小鼠
结核分枝杆菌
的影像学
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Imaging
Mycobacterium tuberculosis
in Mice with Reporter Enzyme Fluorescence
Please note that all translations are automatically generated.
Click here for the English version.
报告酶荧光法检测小鼠
结核分枝杆菌
的影像学
DOI:
10.3791/56801-v
•
10:06 min
•
February 26, 2018
•
Riti Sharan*
1
,
Hee-Jeong Yang*
2
,
Preeti Sule
,
Jeffrey D. Cirillo
1
Department of Microbial Pathogenesis and Immunology
,
Texas A&M Health Science Center
,
2
Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases
,
National Institutes of Health
Chapters
00:04
Title
01:02
Preparing the
M. tuberculosis
Inoculum
03:13
Inoculating Mice with Airborne
M. tuberculosis
05:33
Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
08:05
Results: Imaging
09:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了使用报告酶荧光 (REF) 感染结核分
枝杆菌
(
m 结核
) 的小鼠的光学成像。该协议有助于在临床前动物模型中对
m. 结核
进行敏感和特定的检测, 以进行发病机制、治疗和疫苗研究。
Tags
Reporter Enzyme Fluorescence
Mycobacterium Tuberculosis
Pre-clinical Animal Models
Pathogenesis
Therapeutics
Vaccine Research
Virulence Mechanisms
Host-pathogen Interactions
MOADCTW Medium
7H11 Plates
CFU
Biosafety Hood
Nebulizer
Inoculation Chamber
Article
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