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Imaging Mycobacterium tuberkulose i mus med Reporter enzym fluorescens
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Imaging Mycobacterium tuberkulose i mus med Reporter enzym fluorescens

DOI:
10.3791/56801-v

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10:06 min

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February 26, 2018

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, , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Chapters

  • 00:04Title
  • 01:02Preparing the M. tuberculosis Inoculum
  • 03:13Inoculating Mice with Airborne M. tuberculosis
  • 05:33Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
  • 08:05Results: Imaging
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver den optiske billeddannelse af mus inficeret med Mycobacterium tuberculosis (M. tuberkulose) ved hjælp af reporter enzym fluorescens (REF). Denne protokol letter følsom og specifik påvisning af M. tuberkulose i prækliniske dyremodeller for patogenesen, therapeutics og vaccine forskning.

Tags

Keywords: Reporter Enzyme FluorescenceMycobacterium TuberculosisPre-clinical Animal ModelsPathogenesisTherapeuticsVaccine ResearchVirulence MechanismsHost-pathogen InteractionsMOADCTW Medium7H11 PlatesCFUBiosafety HoodNebulizerInoculation Chamber

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