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Biochemistry

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Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
 

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Article doi: 10.3791/56896
January 30th, 2018

Summary January 30th, 2018

Here, we present a protocol to obtain three-color smFRET data and its analysis with a 3D ensemble Hidden Markov Model. With this approach, scientists can extract kinetic information from complex protein systems, including cooperativity or correlated interactions.

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