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Journal
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Genetics
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使用 Macroarrays 的转移 rna 的代谢标记和剖面分析
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
Please note that all translations are automatically generated.
Click here for the English version.
使用 Macroarrays 的转移 rna 的代谢标记和剖面分析
DOI:
10.3791/56898-v
•
10:56 min
•
January 16, 2018
•
Sophia Emetu*
1
,
Morgan Troiano*
1
,
Jacob Goldmintz*
1
,
Jensen Tomberlin
,
Simon Grelet
,
Philip H. Howe
,
Christopher Korey
,
Renaud Geslain
1
Laboratory of tRNA Biology, Department of Biology
,
College of Charleston
,
2
Department of Biochemistry and Molecular Biology
,
MUSC
,
3
Department of Biology
,
College of Charleston
Chapters
00:05
Title
01:06
Culture and Metabolic Labeling
02:23
Preparation of Total RNAs
04:08
Preparation of 96-well Printer Plates
04:38
Array Printing
07:26
Array Hybridization and Quantification
10:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个原始的传输 RNA 分析平台名为 SPOt (简化的观察 tRNA 平台)。斑点同时测量所有转运在生物样品中的细胞水平, 仅三步, 并在不到24小时。
Tags
Metabolic Labeling
TRNA Profiling
Macroarray Analysis
TRNA Regulation
Protein Synthesis
Mycobacterium Smegmatis
Phosphorus-32 Labeling
RNA Extraction
Chloroform Extraction
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