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Journal / Genetics / Etiquetado metabólico y perfiles de RNAs de…
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Genetics

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JoVE Journal Genetics
Etiquetado metabólico y perfiles de RNAs de transferencia utilizando Macroarrays
Generation and Purification of Chromosome Conformation Capture (3C) Library
Journal (Genetics)
Generation and Purification of Chromosome Conformation Capture (3C) Library
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Journal (Genetics)
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
 
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Etiquetado metabólico y perfiles de RNAs de transferencia utilizando Macroarrays

Article DOI: 10.3791/56898-v • 10:56 min • January 16th, 2018
January 16th, 2018 •
*1, *1, *1, 1, 2, 2, 3, 1
1Laboratory of tRNA Biology, Department of Biology, College of Charleston, 2Department of Biochemistry and Molecular Biology, MUSC, 3Department of Biology, College of Charleston
* These authors contributed equally

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Describimos a una plataforma de análisis de ARN de transferencia original llamada SPOt (plataforma optimizada para la observación de tRNA). Lugar simultáneamente mide los niveles celulares de todos los tRNAs en muestras biológicas, en sólo tres pasos y en menos de 24 horas.

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Genética número 131 ácidos ribonucleicos de transferencia ortofosfato radiactivo macroarrays etiquetado metabólico expresión génica traducción genética síntesis de proteínas ácidos ribonucleicos no codificante
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