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Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

DOI:
10.3791/56911-v

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11:26 min

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February 07, 2018

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, , , , ,
1Neuroscience Program,University of Miami School of Medicine, 2Department of Biology,University of Miami

Chapters

  • 00:04Title
  • 01:03Animal Mounting
  • 02:44Imaging Setup
  • 05:36Ratiometric HSN Ca2 Imaging and C. elegans Behavior Recording
  • 07:09Image Segmentation and Quantitative Analysis
  • 08:58Results: Representative HSN Imaging During Egg-laying
  • 10:35Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of genetically encoded Ca2+ reporters to record changes in neural activity in behaving Caenorhabditis elegans worms.  

Tags

Keywords: Ratiometric Calcium ImagingCaenorhabditis ElegansHSN NeuronBehavioral NeuroscienceGCaMP5MCherryNematode Growth MediumOP50 BacteriaVulvaInverted MicroscopeInfrared FilterVideo AnalysisStage XY Stage Control

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