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比率钙显像对单个神经元的行为线虫线虫

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

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比率钙显像对单个神经元的行为线虫线虫

DOI:

10.3791/56911-v

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11:26 min

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February 07, 2018

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Bhavya Ravi*¹, Layla M. Nassar*^1,2, Richard J. Kopchock III*², Pravat Dhakal*², Michael Scheetz, Kevin M. Collins²

¹Neuroscience Program,University of Miami School of Medicine, ²Department of Biology,University of Miami

Chapters

00:04Title
01:03Animal Mounting
02:44Imaging Setup
05:36Ratiometric HSN Ca² Imaging and C. elegans Behavior Recording
07:09Image Segmentation and Quantitative Analysis
08:58Results: Representative HSN Imaging During Egg-laying
10:35Conclusion

Summary

Automatic Translation

该协议描述了使用基因编码的 Ca²记者记录在行为上的神经活动的变化, 在线虫线虫蠕虫。

Tags

Keywords: Ratiometric Calcium Imaging Caenorhabditis Elegans HSN Neuron Behavioral Neuroscience GCaMP5 MCherry Nematode Growth Medium OP50 Bacteria Vulva Inverted Microscope Infrared Filter Video Analysis Stage XY Stage Control

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