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Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

DOI:
10.3791/56936-v

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10:56 min

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December 21, 2017

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, , , ,
1Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Associazione Annastaccatolisa Onlus, 3Nuclear Medicine Unit,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Chapters

  • 00:05Title
  • 01:34Immuno-DNA FISH (Fluorescent In Situ Hybridization) on Wire
  • 02:35Fixation, Permeabilization, and Immunofluorescence
  • 04:253D DNA FISH Day 2
  • 06:083D DNA FISH Day 3
  • 07:513D Microscope Observation and Analysis
  • 09:01Results: No Interference Bewteen Immunofluorescence and DNA FISH Signals
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

We present a new approach to characterize tumor cells. We combined immunofluorescence with DNA fluorescent-in-situ-hybridization to evaluate cells captured by a functionalized medical wire capable of in vivo enriching CTCs directly from patient blood.

Tags

Keywords: Tumor CellsCirculating Tumor Cells3D ApproachImmunofluorescenceDNA FISHEpCAMCytosomatic AlterationsARC GeneNon-small-cell Lung CancerBreast CancerCTC CharacterizationIn Situ DetectionImmunophenotypingFluorescence Microscopy

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