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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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Cancer Research
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JoVE Journal Cancer Research
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

DOI:
10.3791/56972-v

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10:41 min

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April 05, 2018

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, , , , , , ,
1Department of Genomic Medicine,University of Texas MD Anderson Cancer Center, 2The Jackson Laboratory for Genomic Medicine

Chapters

  • 00:04Title
  • 00:31Tissue/Cell Line Processing and Cross-linking
  • 01:57Tissue Lysis, Sonication, and Antibody Preparation
  • 02:52Chromatin Immunoprecipitation
  • 03:40Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
  • 04:43Purification and Quantification of Precipitated DNA
  • 06:00Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
  • 07:53Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe an optimized high-throughput ChIP-sequencing protocol and computational analyses pipeline for the determination of genome-wide chromatin state patterns from frozen tumor tissues and cell lines.

Tags

Keywords: Chromatin StatesChIP-sequencingCancer EpigeneticsHistone ModificationsChromatin FragmentsHistone AntibodiesProtein G Magnetic BeadsChIP Dilution Buffer

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