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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

DOI:
10.3791/56993-v

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08:54 min

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February 12, 2018

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, , , , , ,
1Department of Biochemistry,University of Nebraska

Chapters

  • 00:04Title
  • 00:45Catheter Placement
  • 02:46Liver Perfusion and Hepatocyte Purification
  • 04:39Sinusoidal Endothelial Cell (SEC) Purification
  • 07:18Results: Representative SEC Purification Analyses
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.

Tags

Keywords: Mouse Liver PerfusionHepatocyte PurificationSinusoidal Endothelial Cell PurificationLiver Cell PopulationsPortal Vein CatheterizationLiver DigestionLiver Cell ViabilityHepatocyte HarvestingBuffer Solutions

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