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JoVE Journal
Biochemistry

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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
 

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Article doi: 10.3791/56993
February 12th, 2018

Summary February 12th, 2018

The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.

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