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Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
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1Department of Otorhinolaryngology,Johannes-Gutenberg University Medical Center, 2Department of Otorhinolaryngology,Technical University of Munich Medical Center, 3Department of Otorhinolaryngology,Ludwig-Maximilian-University Medical Center, 4Department of Otorhinolaryngology,University of Goettingen Medical Center
Chapters
- 00:05Title
- 00:45Processing the Tumor Specimen
- 01:39Seeding the Cells on Ultra-low Adhesion Cell Culture Plates and Exposing Spheroids to Multimodal Standard or Experimental Tumor Therapy
- 02:52Assessment of Spheroid Size and Extent of Cellular Proliferation for Assay Read-out
- 04:08Results: Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
- 05:12Conclusion
We describe the evolution of a spheroid-based, three-dimensional in vitro model that enables us to test the current standard of experimental therapy regimens for head and neck squamous cell carcinoma on cell lines, aiming at evaluating therapy susceptibility and resistance on primary cells from human specimens in the future.
Tags
Keywords:
3D Cell CultureSpheroidHead And Neck Squamous Cell CarcinomaTumor GrowthRadiationChemoradiationPrimary Tumor CellsCollagenaseCell StrainerCell Culture FlaskUltra-low Adhesion 96-well PlateMedia ChangeCisplatin5-FluorouracilSpheroid Size MeasurementDigital Photo DocumentationCentrifugationPBS Wash
