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A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
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Cancer Research
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JoVE Journal Cancer Research
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

DOI:
10.3791/57035-v

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12:48 min

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February 16, 2018

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, ,
1Vascular Biology Program,Boston Children’s Hospital, 2Department of Surgery,Harvard Medical School and Boston Children’s Hospital

Chapters

  • 00:04Title
  • 00:58Cell Preparation
  • 02:35Cell Preparation: Splitting KHOS-A and KHOS-N Cells
  • 03:50Cell Preparation: Seeding KHOS-A and KHOS-N Cells for QPI
  • 04:30Quantitative Phase Image Acquisition: Cover Slip Set-up
  • 05:19Quantitative Phase Imaging
  • 07:03Analyzing Cell Morphology
  • 08:20Analyzing Cell Proliferation
  • 08:54Analyzing Cell Motion
  • 10:16Results: Quantiative Phase Imaging of Dormant and Active Human Cancer Cells
  • 11:50Conclusion

Summary

Automatic Translation

Automatic Translation

Dormant and active cancer cell phenotypes were characterized using quantitative phase imaging. Cell proliferation, migration, and morphology assays were integrated and analyzed in one simple method.

Tags

Keywords: Time-lapseLabel-freeQuantitative Phase ImagingDormant Cancer CellsActive Cancer CellsAngiogenic PhenotypeNon-angiogenic PhenotypeOsteosarcoma CellsCell MorphologyCell ProliferationCell MotilityTumor DormancyEndogenic SwitchMulticellular ParametersCell AreaCell ThicknessCell VolumeProliferation RateDoubling TimeMotility SpeedMigrationCell CultureCell Passaging

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