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用荧光标记示踪剂对小鼠血脑屏障通透性的体内检测
 
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用荧光标记示踪剂对小鼠血脑屏障通透性的体内检测

Article DOI: 10.3791/57038-v 09:35 min February 26th, 2018
February 26th, 2018

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Summary

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在这里, 我们提出了一个小鼠脑血管通透性检测, 采用腹腔注射荧光示踪剂后灌注, 适用于动物模型的血脑屏障功能障碍。一个半脑用于定量评估渗透性, 另一个用于示踪器可视化/染色。10只老鼠的手术过程需要 5-6 小时。

Tags

神经科学 问题 132 血脑屏障 屏障 体内 通透 定量 荧光示踪剂 内皮细胞 微血管 毛细血管 腹腔 灌注
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