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JoVE Journal
Immunology and Infection
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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
 

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Article DOI: 10.3791/57061
December 9th, 2017

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Summary December 9th, 2017

Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.

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