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Neuroscience

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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
 

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Article doi: 10.3791/57065
February 16th, 2018 Usage Statistics

Summary February 16th, 2018

A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.

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