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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
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JoVE Journal Biology
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

DOI:
10.3791/57127-v

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08:54 min

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February 14, 2018

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, , , , , ,
1Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences,University of Brasília, 2Department of Microbiology and Immunology,Albert Einstein College of Medicine, 3Division of Infectious Diseases, Department of Medicine,Albert Einstein College of Medicine, 4Faculty of Ceilândia,University of Brasília

Chapters

  • 00:04Title
  • 00:47Preparing Fungal Inoculum and Peptides
  • 02:48Antifungal Assay
  • 05:23Results: Detection of New Antimicrobial Peptides
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.

Tags

Keywords: Broth MicrodilutionIn Vitro ScreeningAntifungal CompoundsFungal ActivityRPMI 1640 MediumC. AlbicansC. NeoformansPeptidesSerial Dilution96-well Microplate

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