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A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae
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Behavior
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JoVE Journal Behavior
A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae

A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae

DOI:
10.3791/57131-v

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09:04 min

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March 27, 2018

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, ,
1Department of Biosciences,Rice University, 2Yale Medical School

Chapters

  • 00:04Title
  • 01:03Preparation of the Larvae and Assay Plates
  • 02:52Conducting the Assay
  • 04:24Preparing Assay Plates for Imaging
  • 07:06Results: Assessing RNAi Suppression of uninflatable
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes a simple assay to identify Drosophila melanogaster larvae that are experiencing hypoxia under normal atmospheric oxygen levels. This protocol allows hypoxic larvae to be distinguished from other mutants that show overlapping phenotypes such as sluggishness or slow growth.

Tags

Keywords: Burrowing AssayTunneling AssayHypoxia DetectionDrosophila Melanogaster LarvaeOxygen DeficiencyLarval BehaviorTracheaWandering PhaseGenetic CrossesEgg CollectionAgar PlatesNipagenYeast PasteCork Board

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