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Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
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JoVE Journal Neuroscience
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

DOI:
10.3791/57139-v

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07:13 min

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January 07, 2019

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, ,
1Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital,Mass General Institute of Neurodegenerative Disease, 2Department of Geriatric Neurology, Nanlou Clinical Division,PLA General Hospital

Chapters

  • 00:04Title
  • 01:01Larvae Collection and Dissection
  • 02:57Imaging Processing
  • 05:32Results: Silencing Sox102F Affects Dendrite Size
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol focuses on quantitative analysis of neuronal dendritic arborization complexity (NDAC) in Drosophila, which can be used for studies of dendritic morphogenesis.

Tags

Keywords: Dendritic ArborizationDrosophilaSOX5 GeneNeuro-dendritesMorphogenesisNeurodegenerative DiseaseUAS-GFPPpk-GAL4UAS-Sox102F-RNAiThird Instar LarvaeDissectionPBSPFAAntifade Mounting MediumConfocal MicroscopyFiji ImageJDendrite Length

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