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JoVE Journal Chemistry
Absorción de Metal esencial en bacterias Gram-negativas
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Absorción de Metal esencial en bacterias Gram-negativas: rayos x de fluorescencia, radioisótopos y fraccionamiento de la célula

Article DOI: 10.3791/57169-v • 10:34 min • February 1st, 2018
February 1st, 2018 •
1, 2, 2, 3, 2, 4
1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

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Un protocolo para la extracción de una chaperona de periplasmic metales de transición en el contexto de sus socios de Unión nativos y caracterización biofísica de su contenido de sustrato mediante rayos x se presenta absorción fluorescencia y radiometal.

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Química número 132 fraccionamiento periplasma fluorescencia de rayos x Metal de la transición proteína de unión a sustrato (SBP) YfeA Yersinia pestis peste radiosonda radiactividad
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