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Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions
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Environment
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JoVE Journal Environment
Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

DOI:
10.3791/57170-v

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11:57 min

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April 10, 2018

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, , , , , , , , ,
1Environmental Genomics and Systems Biology Division,Lawrence Berkeley National Laboratory, 2Joint Genome Institute,Department of Energy, 3Joint BioEnergy Institute, 4Department of Environmental Science Policy and Management,University of California

Chapters

  • 00:04Title
  • 01:13EcoFAB Device Fabrication: Casting PDMS Layers
  • 03:22EcoFAB Device Fabrication: Chemically Attached PDMS Layers onto Microscope Slides
  • 04:50EcoFAB Sterilization
  • 05:59Growing Plants in EcoFABs
  • 08:31Results: Plant-microbe Interactions Using Ecosystem Fabrication Protocols
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes detailed protocols for ecosystem fabrication of devices (EcoFABs) that enable the studies of plants and plant-microbe interactions in highly controlled laboratory conditions.

Tags

Keywords: Ecosystem Fabrication (EcoFAB)Plant-microbe InteractionsLaboratory Ecosystems3D PrintingPDMSMicroscope SlidesRoot MorphologyMass Spectroscopic ImagingSustainable Agriculture

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