Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

This method can help answer key questions in oral cancer research field, such as how to build an appropriate T-assist model in-vitro. The main advantage of this technique is that reconstructed TSCC express tumor characteristics that bear a strong resemblance to clinical TSCC histopathology. When first had the idea for this method when we realized that 2-D cultured TSCC cells did not properly exhibit the native phenotype of TSCC.

Furthermore, we realized the importance of extracellular matrix for cancer survival evasion and differentiation. Visual demonstration of this method is critical as the preparation of tongue extracellular matrix and 3-D culture using a mini bioreactor are difficult to learn through words only, and are best as seen for clarification. After isolating the tongues from euthanized mice according to the text protocol, immerse the tissue in 75%ethanol for three minutes.