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Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
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Cancer Research
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JoVE Journal Cancer Research
Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

DOI:
10.3791/57235-v

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08:15 min

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June 20, 2018

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, ,
1Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences,Sun Yat-sen University

Chapters

  • 00:04Title
  • 01:21Preparation of Extracellular Matrix
  • 04:11Static TSCC Model Reconstruction and Preparation of Self-made Stirred Minibioreactor and Dynamic Cell Culture
  • 06:28Results: Reconstitution of Tongue Squamous Cell Carcinoma
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

A method is shown here for the preparation of the tongue extracellular matrix (TEM) with efficient decellularization. The TEM can be used as functional scaffolds for the reconstruction of a tongue squamous cell carcinoma (TSCC) model under static or stirred culture conditions.

Tags

Keywords: Tongue Extracellular MatrixTongue Squamous Cell CarcinomaIn Vitro3D CultureBioreactorOral Cancer Research

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