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March 10, 2018
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A flow cytometry-based cellular binding assay is described that is primarily used as a screening tool to identify compounds that inhibit the binding of a fluorescently labeled CXC chemokine ligand 12 (CXCL12) to the CXC chemokine receptor 4 (CXCR4).
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Cite this Article
Schoofs, G., Van Hout, A., D'huys, T., Schols, D., Van Loy, T. A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4. J. Vis. Exp. (133), e57271, doi:10.3791/57271 (2018).
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