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En flyt cytometri-baserte analysen identifisere forbindelser som forstyrrer Binding av Fluorescently-merket CXC Chemokine Ligand 12 til CXC Chemokine reseptor 4
JoVE Journal
Biology
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JoVE Journal Biology
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
DOI:

06:56 min

March 10, 2018

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Chapters

  • 00:04Title
  • 00:54Competition Binding Assay
  • 03:00Analysis of the Samples by Flow Cytometry
  • 05:35Results: Evaluation and Analysis of Active and Inactive Compounds in the Competition Binding Assay
  • 06:35Conclusion

Summary

Automatic Translation

En flyt cytometri-baserte mobilnettet bindende analysen er beskrevet som hovedsakelig brukes som en screening verktøyet for å identifisere forbindelser som hemmer binding av en fluorescently merket CXC chemokine ligand 12 (CXCL12) til CXC chemokine reseptoren 4 (CXCR4).

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