Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

The overall goal of this procedure is to create a transgenic zebrafish line that allows the observance of cellular electrical changes during embryogenesis, larval movement, and in tumor genesis. This method could help answer key questions in the developmental biology, physiology, and cancer cell biology fields such as what are the fundamental roles of cellular electrical signaling during embryogenesis and in tumor cells? The main advantage of this technique is that it allows us to track cellular electrical signaling in vivo and in real time.

After preparing Tol2 transposase mRNA and injection solution according to the text protocol, the afternoon before injection, set up four to six breeding tanks with at least two males and two females. To reduce the amount of fish waste and to induce a breeding response, avoid feeding the fish in the afternoon. The following morning, remove the prepared injection solution from the negative 80 degree Celsius freezer and place it on ice.