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Immunology and Infection

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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Article DOI: 10.3791/57357-v • 12:36 min • May 1st, 2018
May 1st, 2018 •
*1, *1, 2,3, 1,2,3, 1, 1
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.

Tags

Synthetic Phage Displayed Fab Library Tailored Diversity Antibody Library Hybridoma-based Techniques Therapeutic Monoclonal Antibody Development Animal Immunization Antibody Humanization Positional Diversity High Affinity Interaction Protein Properties Amino Acids Biomolecular Interactions Graduate Student Doctor Shin-chen Hou Monoclonal Antibodies (mAbs) Hybridoma Technology Phage Display Methods
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