Sachdev S. Sidhu

The Recombinant Antibody Network

Affiliated withThe Recombinant Antibody Network University of Toronto ShanghaiTech University

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Biography

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JoVE Journal Publications

ArticleTotal : 2	Year
Publication title Cited by 33	2015
Publication title Cited by 7	2018

Other Publications

Article	Year
The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF. The Journal of biological chemistry\| PubMed ID: 11821434	2002
Rapid identification of small binding motifs with high-throughput phage display: discovery of peptidic antagonists of IGF-1 function. Chemistry & biology\| PubMed ID: 11983338	2002
Comprehensive functional maps of the antigen-binding site of an anti-ErbB2 antibody obtained with shotgun scanning mutagenesis. Journal of molecular biology\| PubMed ID: 12079396	2002
A minimized M13 coat protein defines the requirements for assembly into the bacteriophage particle. Journal of molecular biology\| PubMed ID: 12217696	2002
Origins of PDZ domain ligand specificity. Structure determination and mutagenesis of the Erbin PDZ domain. The Journal of biological chemistry\| PubMed ID: 12446668	2003
Exploring protein-protein interactions with phage display. Chembiochem : a European journal of chemical biology\| PubMed ID: 12512072	2003
Functional genomics of intracellular peptide recognition domains with combinatorial biology methods. Current opinion in chemical biology\| PubMed ID: 12547433	2003
The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affini... Journal of molecular biology\| PubMed ID: 12946357	2003
Contributions of CDR3 to V H H domain stability and the design of monobody scaffolds for naive antibody libraries. Journal of molecular biology\| PubMed ID: 12963373	2003
Comprehensive mutagenesis of the C-terminal domain of the M13 gene-3 minor coat protein: the requirements for assembly into the bacteriophage particle. Journal of molecular biology\| PubMed ID: 12972250	2003
Definition of the consensus motif recognized by gamma-adaptin ear domains. The Journal of biological chemistry\| PubMed ID: 14665628	2004
Bivalent antibody phage display mimics natural immunoglobulin. Journal of immunological methods\| PubMed ID: 14736422	2004
Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions. Journal of molecular biology\| PubMed ID: 15066433	2004
Comprehensive mutational analysis of the M13 major coat protein: improved scaffolds for C-terminal phage display. Journal of molecular biology\| PubMed ID: 15210356	2004
High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold. Journal of molecular biology\| PubMed ID: 15236968	2004
Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 15306681	2004
Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis. Journal of molecular biology\| PubMed ID: 15755445	2005
A structure-based database of antibody variable domain diversity. Journal of molecular biology\| PubMed ID: 15826665	2005
Molecular recognition by a binary code. Journal of molecular biology\| PubMed ID: 15854651	2005
Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis. Protein science : a publication of the Protein Society\| PubMed ID: 16131663	2005
Tyrosine plays a dominant functional role in the paratope of a synthetic antibody derived from a four amino acid code. Journal of molecular biology\| PubMed ID: 16413576	2006
Convergent and divergent ligand specificity among PDZ domains of the LAP and zonula occludens (ZO) families. The Journal of biological chemistry\| PubMed ID: 16737968	2006
Comparative structural analysis of the Erbin PDZ domain and the first PDZ domain of ZO-1. Insights into determinants of PDZ domain specificity. The Journal of biological chemistry\| PubMed ID: 16737969	2006
Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning. The Journal of biological chemistry\| PubMed ID: 16762925	2006
Activation of the proapoptotic death receptor DR5 by oligomeric peptide and antibody agonists. Journal of molecular biology\| PubMed ID: 16859704	2006
M13 bacteriophage coat proteins engineered for improved phage display. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 17041267	2007
Synthetic therapeutic antibodies. Nature chemical biology\| PubMed ID: 17108986	2006
Full-length antibodies on display. Nature biotechnology\| PubMed ID: 17483836	2007
Exploring and designing protein function with restricted diversity. Current opinion in chemical biology\| PubMed ID: 17500026	2007
Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries. Nature protocols\| PubMed ID: 17545975	2007
Structural and functional analysis of the ligand specificity of the HtrA2/Omi PDZ domain. Protein science : a publication of the Protein Society\| PubMed ID: 17656586	2007
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries. Journal of molecular biology\| PubMed ID: 17825836	2007
Phage display for engineering and analyzing protein interaction interfaces. Current opinion in structural biology\| PubMed ID: 17870470	2007
The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways. The Journal of cell biology\| PubMed ID: 17893247	2007
Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein science : a publication of the Protein Society\| PubMed ID: 17962403	2007
Comprehensive analysis of the factors contributing to the stability and solubility of autonomous human VH domains. The Journal of biological chemistry\| PubMed ID: 18045863	2008
Synthetic antibodies for specific recognition and crystallization of structured RNA. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 18162543	2008
Regulation of a late phase of T cell polarity and effector functions by Crtam. Cell\| PubMed ID: 18329370	2008
The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies. Journal of molecular biology\| PubMed ID: 18336836	2008
Submitting antibodies to binding arbitration. Nature chemical biology\| PubMed ID: 18488004	2008
A dominant conformational role for amino acid diversity in minimalist protein-protein interfaces. Journal of molecular biology\| PubMed ID: 18602117	2008
Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies. Cell\| PubMed ID: 18724939	2008
A specificity map for the PDZ domain family. PLoS biology\| PubMed ID: 18828675	2008
Two-state selection of conformation-specific antibodies. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 19208804	2009
Inhibition of Wnt signaling by Dishevelled PDZ peptides. Nature chemical biology\| PubMed ID: 19252499	2009
The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins. ACS chemical biology\| PubMed ID: 19298050	2009
Crystal structure of full-length KcsA in its closed conformation. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 19346472	2009
Rapid evolution of functional complexity in a domain family. Science signaling\| PubMed ID: 19738200	2009
Improving therapeutic efficacy of a complement receptor by structure-based affinity maturation. The Journal of biological chemistry\| PubMed ID: 19833734	2009
Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins. PLoS biology\| PubMed ID: 19841731	2009
The functional capacity of the natural amino acids for molecular recognition. Molecular bioSystems\| PubMed ID: 20383388	2010
MOTIPS: automated motif analysis for predicting targets of modular protein domains. BMC bioinformatics\| PubMed ID: 20459839	2010
Structure of the complex between HER2 and an antibody paratope formed by side chains from tryptophan and serine. Journal of molecular biology\| PubMed ID: 20654626	2010
Coevolution of PDZ domain-ligand interactions analyzed by high-throughput phage display and deep sequencing. Molecular bioSystems\| PubMed ID: 20714644	2010
Functional complexes between YAP2 and ZO-2 are PDZ domain-dependent, and regulate YAP2 nuclear localization and signalling. The Biochemical journal\| PubMed ID: 20868367	2010
A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination. Nature structural & molecular biology\| PubMed ID: 21151117	2011
Evolving specificity from variability for protein interaction domains. Trends in biochemical sciences\| PubMed ID: 21227701	2011
Development of reagents and assays for the detection of pathogenic Burkholderia species. Faraday discussions\| PubMed ID: 21413172	2011
The multiple-specificity landscape of modular peptide recognition domains. Molecular systems biology\| PubMed ID: 21525870	2011
Phage display of tissue inhibitor of metalloproteinases-2 (TIMP-2): identification of selective inhibitors of collagenase-1 (metalloproteinase 1 (MMP-1)). The Journal of biological chemistry\| PubMed ID: 21715326	2011
Identification of specificity determining residues in peptide recognition domains using an information theoretic approach applied to large-scale binding maps. BMC biology\| PubMed ID: 21835011	2011
Studying binding specificities of peptide recognition modules by high-throughput phage display selections. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 21877279	2011
Simplified synthetic antibody libraries. Methods in enzymology\| PubMed ID: 22208979	2012
MUSI: an integrated system for identifying multiple specificity from very large peptide or nucleic acid data sets. Nucleic acids research\| PubMed ID: 22210894	2012
Charged and hydrophobic surfaces on the a chain of shiga-like toxin 1 recognize the C-terminal domain of ribosomal stalk proteins. PloS one\| PubMed ID: 22355345	2012
Synthetic antibodies as tools to probe RNA-binding protein function. Molecular bioSystems\| PubMed ID: 22481296	2012
Synthetic human monoclonal antibodies toward staphylococcal enterotoxin B (SEB) protective against toxic shock syndrome. The Journal of biological chemistry\| PubMed ID: 22645125	2012
Antibodies for all: The case for genome-wide affinity reagents. FEBS letters\| PubMed ID: 22664378	2012
Elucidation of the binding preferences of peptide recognition modules: SH3 and PDZ domains. FEBS letters\| PubMed ID: 22691579	2012
Recombinant antibodies and in vitro selection technologies. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22723092	2012
Synthetic antibody libraries. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22735944	2012
Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation. Science (New York, N.Y.)\| PubMed ID: 22878499	2012
Selecting and purifying autonomous human variable heavy (VH) domains. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 22886261	2012
T cell receptor-like recognition of tumor in vivo by synthetic antibody fragment. PloS one\| PubMed ID: 22916301	2012
Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 22927390	2012
Superbinder SH2 domains act as antagonists of cell signaling. Science signaling\| PubMed ID: 23012655	2012
A short DNA aptamer that recognizes TNFα and blocks its activity in vitro. ACS chemical biology\| PubMed ID: 23046187	2013
Two synthetic antibodies that recognize and neutralize distinct proteolytic forms of the ebola virus envelope glycoprotein. Chembiochem : a European journal of chemical biology\| PubMed ID: 23111988	2012
Immunology: Evolving superkines for selectivity. Nature chemical biology\| PubMed ID: 23183579	2012
KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression. The Journal of cell biology\| PubMed ID: 23209302	2012
CDR-H3 diversity is not required for antigen recognition by synthetic antibodies. Journal of molecular biology\| PubMed ID: 23219464	2013
A strategy for modulation of enzymes in the ubiquitin system. Science (New York, N.Y.)\| PubMed ID: 23287719	2013
Engineering and analysis of peptide-recognition domain specificities by phage display and deep sequencing. Methods in enzymology\| PubMed ID: 23422437	2013
Interaction domains of Sos1/Grb2 are finely tuned for cooperative control of embryonic stem cell fate. Cell\| PubMed ID: 23452850	2013
Design of synthetic autonomous VH domain libraries and structural analysis of a VH domain bound to vascular endothelial growth factor. Journal of molecular biology\| PubMed ID: 23507309	2013
SH3 interactome conserves general function over specific form. Molecular systems biology\| PubMed ID: 23549480	2013
Engineering ubiquitin to modulate the ubiquitin proteosome system. Cell cycle (Georgetown, Tex.)\| PubMed ID: 23673323	2013
Targeting HER2+ breast cancer cells: lysosomal accumulation of anti-HER2 antibodies is influenced by antibody binding site and conjugation to polymeric nanoparticles. Journal of controlled release : official journal of the Controlled Release Society\| PubMed ID: 23880472	2013
Recombinant genetic libraries and human monoclonal antibodies. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 24037841	2014
Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 24127596	2013
Development of inhibitors in the ubiquitination cascade. FEBS letters\| PubMed ID: 24239534	2014
Peptide binding properties of the three PDZ domains of Bazooka (Drosophila Par-3). PloS one\| PubMed ID: 24466078	2014
Design and generation of synthetic antibody libraries for phage display. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 24515463	2014
Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 24550280	2014
Synthetic antibody technologies. Current opinion in structural biology\| PubMed ID: 24721448	2014
Prediction and experimental characterization of nsSNPs altering human PDZ-binding motifs. PloS one\| PubMed ID: 24722214	2014
Alteration of the C-terminal ligand specificity of the erbin PDZ domain by allosteric mutational effects. Journal of molecular biology\| PubMed ID: 24813123	2014
RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology\| PubMed ID: 24997600	2014
Visualization of arrestin recruitment by a G-protein-coupled receptor. Nature\| PubMed ID: 25043026	2014
Synthetic antibodies with a human framework that protect mice from lethal Sudan ebolavirus challenge. ACS chemical biology\| PubMed ID: 25140871	2014
A structural portrait of the PDZ domain family. Journal of molecular biology\| PubMed ID: 25158098	2014
A systematic approach to identify novel cancer drug targets using machine learning, inhibitor design and high-throughput screening. Genome medicine\| PubMed ID: 25165489	2014
The demonstration of αKlotho deficiency in human chronic kidney disease with a novel synthetic antibody. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association\| PubMed ID: 25324355	2014
The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast. Molecular biology of the cell\| PubMed ID: 25428987	2015
MicroPET/CT imaging of patient-derived pancreatic cancer xenografts implanted subcutaneously or orthotopically in NOD-scid mice using (64)Cu-NOTA-panitumumab F(ab')2 fragments. Nuclear medicine and biology\| PubMed ID: 25456837	2015
Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition. Genome biology\| PubMed ID: 25962635	2015
A High Through-put Platform for Recombinant Antibodies to Folded Proteins. Molecular & cellular proteomics : MCP\| PubMed ID: 26290498	2015
A switchable yeast display/secretion system. Protein engineering, design & selection : PEDS\| PubMed ID: 26333274	2015
Rapid isolation of peptidic inhibitors of the solute carrier family transporters OATP1B1 and OATP1B3 by cell-based phage display selections. Biochemical and biophysical research communications\| PubMed ID: 26792727	2016
System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes. Molecular cell\| PubMed ID: 26949039	2016
Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1-F-box interface. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 26976582	2016
Saturation scanning of ubiquitin variants reveals a common hot spot for binding to USP2 and USP21. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 27436899	2016
Engineering cell signaling modulators from native protein-protein interactions. Current opinion in structural biology\| PubMed ID: 27866084	2017
A Highly Diverse and Functional Naïve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents. Journal of molecular biology\| PubMed ID: 27887869	2017
Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 28092036	2017
Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds. Frontiers in immunology\| PubMed ID: 28184223	2017
Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10. Journal of molecular biology\| PubMed ID: 28587923	2017
Comprehensive Analysis of the Human SH3 Domain Family Reveals a Wide Variety of Non-canonical Specificities. Structure (London, England : 1993)\| PubMed ID: 28890361	2017
A General Strategy for Discovery of Inhibitors and Activators of RING and U-box E3 Ligases with Ubiquitin Variants. Molecular cell\| PubMed ID: 29053960	2017
Construction of Synthetic Antibody Phage-Display Libraries. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 29116499	2018