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Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
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Chapters
- 00:05Title
- 00:55Set-up and Sterilization of Spinner Flasks, Recombinant Collagen I Peptide (RCP) Microcarriers, and Bioreactor
- 02:09Culture of hBMSCs and HDMECs on RCP Macroporous Microcarriers
- 03:29Biomatrix Seeding and Start of Bioreactor System for HDMECs
- 05:05Addition of RFP-HDMECs to the Biomatrix
- 06:16Results: Cell Expansion on Macroporous Microcarriers and Cell Delivery to a Biomatrix in a Bioreactor Model
- 07:19Conclusion
We propose a cell expansion protocol on macroporous microcarriers and their use as delivery system in a perfusion bioreactor to seed a decellularized tissue matrix. We also include different techniques to determine cell proliferation and viability of cells cultured on microcarriers. Furthermore, we demonstrate functionality of cells after bioreactor cultures.
