Journal
/
Biology
/
高通量 SiRNA 筛选氯化苦和氟化氢引起的角膜上皮细胞损伤
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury
Please note that all translations are automatically generated. Click here for the English version.

高通量 SiRNA 筛选氯化苦和氟化氢引起的角膜上皮细胞损伤

DOI:
10.3791/57372-v

•

14:20 min

•

June 16, 2018

•

, , ,
1US Army Medical Research Institute of Chemical Defense

Chapters

  • 00:00Title
  • 00:23Plate cells for experimentation
  • 02:02Transfection
  • 04:37Positive control addition
  • 05:41HF exposure of cultured cells
  • 07:32Sample collection and cell viability assay
  • 10:04Measure IL-8 concentration in cell culture supernatants
  • 13:29Conclusion

Summary

Automatic Translation

Automatic Translation

高通量小抑制 RNA 筛选是一种重要的工具, 有助于更迅速地阐明化学角膜上皮损伤的分子机制。本文对氟化氢-氯化苦诱导的角膜上皮损伤进行高通量筛选的暴露模型和方法进行了开发和验证。

Tags

High ThroughputSiRNA ScreeningCornea Epithelial CellToxicant InjuryAutomated Liquid HandlingCell SeedingCell Density EvaluationSiRNA Transfection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

高通量筛选和生物传感与荧光 C。线虫菌株

高通量酵母质粒过表达屏幕

真菌内切葡聚糖酶的活性高通量筛选大肠埃希氏菌</em

内向整流钾通道的小分子调节剂的高通量筛选

使用自制双辉光萤光素酶检测的高通量筛选功能

恢复成年斑马鱼的心脏的高通量应用

高通量筛选转录后调控基因的化学调节剂

激酶底物对鉴定使用高通量筛选

多酶筛选使用高通量基因酶的筛选系统

基于蛋白质的继承在酿酒酵母中的高通量筛选

Read Article