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Immunology and Infection
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Visual and Microscopic Evaluation of Streptomyces Developmental Mutants
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

DOI:
10.3791/57373-v

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08:42 min

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September 12, 2018

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, , ,
1Department of Biology and Earth Science, Biochemistry and Molecular Biology Program,Otterbein University, 2Department of Biological Sciences,Duquesne University

Chapters

  • 00:04Title
  • 01:19Propagation of Streptomyces and Comparing and Recording Visual Appearance
  • 03:02Phase-contrast Microscopy
  • 04:40Fluorescence Microscopy
  • 06:10Results: Visual and Microscopic Analysis of Streptomyces Developmental Mutants
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present protocols for novice researchers to initiate phenotyping for the pharmacologically important bacterial genus Streptomyces.

Tags

StreptomycesPhenotypic ObservationVisual CharacterizationMicroscopic ExaminationBacterial PropagationStorageStreptomyces CoelicolorActinomycesHigh School ClassroomUndergraduate Teaching LaboratoryColony MorphologyMutagenesisWild TypeMutant StrainsAgar Plate StreakingIncubationSterile TechniqueContamination Prevention

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