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Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
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Neuroscience
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JoVE Journal Neuroscience
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

DOI:
10.3791/57382-v

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08:41 min

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May 12, 2018

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, , ,
1Institute of Pharmacology and Toxicology,University of Zurich, 2Institute of Pharmaceutical Sciences,Swiss Federal Institute (ETH) Zurich

Chapters

  • 00:04Title
  • 00:42Exposure, Identification, and Fixation of the Vertebrae at the Lumbar Spinal Cord Level
  • 04:08Preparation of the Injection Syringe
  • 05:25Intraspinal Injections
  • 07:12Results: Intraspinal Injection of AAV1-eGFP/AAV1-flex-eGFP
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.

Tags

Keywords: Adeno-associated VirusTransgene ExpressionGenetically Defined NeuronsSpinal CordPain PerceptionIntraspinal DeliveryStereotaxic SurgeryVertebrae Identification

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