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Neuroscience

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Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
 

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Article doi: 10.3791/57382
May 12th, 2018 Usage Statistics

Summary May 12th, 2018

Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.

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