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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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Genetics
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JoVE Journal Genetics
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

DOI:
10.3791/57386-v

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09:00 min

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May 02, 2018

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, ,
1Department of Animal Health,Universidad Complutense de Madrid, 2Visavet Health Surveillance Centre,Universidad Complutense de Madrid, 3Department of Zoology,University of Oxford, 4Department of Microbiology,Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

Chapters

  • 00:05Title
  • 00:33Construction of the Experimental System Encoding the Antibiotic Resistance Gene
  • 04:19Evolutionary Rescue Approach to Experimentally Evolve Antibiotic Resistance
  • 06:14Molecular Basis of the Evolution of Antibiotic Resistance
  • 07:05Results: Study of Evolutionary Rescue and Molecular Basis Underlying the Plasmid Mediated Evolution using DNA Sequencing
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

Tags

Keywords: Antibiotic ResistanceMulticopy PlasmidsExperimental MethodMicrobiologyBacterial EvolutionMolecular BiologyPCRIsothermal AssemblyElectroporationAntibiotic SelectionDNA SequencingPlasmid Construction

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