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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

DOI:
10.3791/57390-v

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10:14 min

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May 04, 2018

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, , ,
1Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service,United States Department of Agriculture, 2Horticultural Research Laboratory, Agricultural Research Service,United States Department of Agriculture

Chapters

  • 00:05Title
  • 00:57dsRNA Delivery via Green Beans
  • 04:05Foliar Spray Application in Large Potted Citrus Trees and Seedlings
  • 05:06Soil/Root Drench Application in Potted Citrus Trees and Seedlings
  • 05:58Stem Tap Application in Large Potted Citrus Trees and Seedlings
  • 07:06dsRNA Treated Clay Granules for Delivery to Insects Through Soil
  • 08:36Results: Successful Induction of RNAi via dsRNA
  • 09:16Conclusion

Summary

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This article demonstrates novel techniques developed for oral delivery of double-stranded RNA (dsRNA) through the vascular tissues of plants for RNA interference (RNAi) in phloem sap feeding insects.

Tags

Keywords: RNA InterferenceDsRNA DeliveryPhloemPlant-sap-feeding InsectsHemipteran PestsBrown Marmorated Stink BugOral DeliveryGene SilencingMolecular BiopesticidesCapillary ActionGreen Beans4th Instar Nymphs

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