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Journal
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Cancer Research
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正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma – An Improved Protocol
Please note that all translations are automatically generated.
Click here for the English version.
正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议
DOI:
10.3791/57400-v
•
10:54 min
•
April 04, 2018
•
Elisabeth Naschberger
,
Daniela Regensburger
,
Clara Tenkerian
,
Melanie Langheinrich
,
Felix B. Engel
,
Carol Geppert
,
Arndt Hartmann
,
Robert Grützmann
,
Vera S. Schellerer*
2
,
Michael Stürzl*
1
1
Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen
,
Friedrich-Alexander University of Erlangen-Nuremberg
,
2
Department of Surgery, University Medical Center Erlangen
,
Friedrich-Alexander University of Erlangen-Nuremberg
,
3
Division of Nephropathology, Department of Pathology, University Medical Center Erlangen
,
Friedrich-Alexander University of Erlangen-Nuremberg
,
4
Department of Pathology, University Medical Center Erlangen
,
Friedrich-Alexander University of Erlangen-Nuremberg
Chapters
00:04
Title
00:51
Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
04:17
FACS Sorting, Harvesting and Characterization of Endothelial Cells
07:17
Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肿瘤内皮细胞是肿瘤微环境和病程的重要决定因素。本文介绍了一种从人大肠癌和正常结肠分离纯化和存活的内皮细胞的协议, 用于药物检测和病机研究。
Tags
Endothelial Cells
Colorectal Carcinoma
Tumor Biology
Angiogenesis
Cell Isolation Protocol
HBSS Buffer
DMEM Medium
Cell Strainer
Centrifugation
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