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Journal / Cancer Research / Aislamiento de células endoteliales humanas de…
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Cancer Research

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JoVE Journal Cancer Research
Aislamiento de células endoteliales humanas de Colon Normal y el Carcinoma colorrectal - un protocolo mejorado
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Aislamiento de células endoteliales humanas de Colon Normal y el Carcinoma colorrectal - un protocolo mejorado

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Las células endoteliales tumorales son determinantes importantes del microambiente tumoral y el curso de la enfermedad. Aquí, se describe un protocolo para el aislamiento de las células endoteliales puras y viables de carcinoma colorrectal humano y colon normal para ser utilizado en la investigación de drogas pruebas y patogenesia.

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Investigación de cáncer número 134 células endoteliales buque Carcinoma colorrectal Tumor Colon Normal CD31 VE-cadherina CD105 clasificación de FACS disociación de tejidos microambiente tumoral fibroblastos
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