Journal
/
Bioengineering
/
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

DOI:
10.3791/57451-v

•

09:58 min

•

April 09, 2018

•

, , ,
1Department of Cardiovascular and Renal Research, Institute of Molecular Medicine,University of Southern Denmark, 2Department of Biochemistry and Molecular Biology,University of Southern Denmark, 3Department of Cardiac, Thoracic and Vascular Surgery,Odense University Hospital

Chapters

  • 00:04Title
  • 01:02Imaging of the Artery Using TPEM
  • 02:47Diameter and Wall Thickness Measurement
  • 03:33Imaging the Collagen and Elastin Microarchitecture
  • 05:10Image Analysis Internal Elastic Lamina Branching Angles
  • 05:51Image Analysis – Collagen Fiber Straightness
  • 07:46Results: Measurement of the Microstructural Changes in Adventitial Collagen and the Internal Elastic Lamina in Response to Vessel Pressure
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.

Tags

Keywords: CollagenElastinMicroarchitectureResistance ArteriesLabel-free Fluorescence MicroscopyTwo-photon MicroscopyExtracellular MatrixVascular MechanicsArtery PressurizationSecond Harmonic GenerationAutofluorescenceFiji Image Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57451?article_type=v

Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study
check_url/57451?article_type=v

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
check_url/57451?article_type=v

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
check_url/57451?article_type=v

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
check_url/57451?article_type=v

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
check_url/57451?article_type=v

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
check_url/57451?article_type=v

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
check_url/57451?article_type=v

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
check_url/57451?article_type=v

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
check_url/57451?article_type=v

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Read Article