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Chapters
- 00:04Title
- 01:07Isolation of Primary Mammary Cell Populations
- 04:07Purification of Mammary Epithelial Cells by FACS and Seeding of Primary Mammary LD Cells
- 05:05Induction of YAP-induced Mammary Stem Cells and Sub-culturing of yMaSCs
- 07:33Generation of yDucts
- 09:57Results: Reprogramming Primary Mammary LD Epithelial Cells into Mammary Stem Cells
- 11:25Conclusion
Availability of somatic SCs is crucial for regenerative medicine, disease modeling and to gain insight into SC properties. Here we present experimental strategies to reprogram, in vitro, differentiated adult cells into their corresponding expandable tissue-specific stem/progenitor cells by the transient expression of the single transcriptional co-activator YAP.
